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Updated: Jul 2, 2026

In Vitro Culture for H5N1-Specific Duck T Cells and Detection of Immune Responses Using Intracellular Cytokine Staining Method
Published on: May 30, 2025
Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures
K Takase1, F Nonaka, M Sakaguchi
1The Chemo-Sero-Therapeutic Research Institute, Shimizu-cho, Kumamoto, Japan.
Abstract:
Five cytopathic viruses morphologically resembling rotaviruses were isolated from duck faeces in chicken kidney cell cultures using conventional methods. One of the isolates, designated F-29 strain, was identified as an avian rotavirus from the following: ribonucleic acid in the viral core, virus growth in the cytoplasm, resistance to chloroform, acid and heating, partial stabilisation to molar magnesium chloride and rotavirus-like morphology by electron microscopy. By immunofluorescence, F-29 strain did not react with porcine and bovine rotaviruses. Neutralising antibodies to the F-29 strain were detected in sera collected from a conventional duck flock, but not from specific-pathogen-free duck flocks.

