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Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Microcomputer-assisted morphometric analysis of airsacculitis caused by Mycoplasma gallisepticum in immunised and
O J Fletcher1, S E Fairchild, F G Smith
1Department of Avian Medicine, Poultry Disease Research Center, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Abstract:
Inflammation caused by inoculation of Mycoplasma gallisepticum (MG) into the air sacs was compared in chickens previously exposed by intramuscular injection of MG with that produced in nonimmunised chickens. Air sacs from chickens inoculated with Frey's media, from those receiving only intramuscular injections of MG, and from nonimmunised non-challenged birds were included in the evaluations. The airsacculitis produced in immunised chickens was more severe at 48 hours post-challenge infection than that produced in nonimmune chickens. At 96 hours after challenge, the airsacculitis in nonimmunised chickens was significantly more severe than that produced in chickens of any other experimental group. The protective effect of immunisation at 96 hours was demonstrated clearly by using the techniques of ring-stabilisation for collection, processing, and embedding of air sacs and microcomputer-assisted morpho-metric analysis. Air sac wall thickness and area measurements correlated positively with subjective impressions on the degree of airsacculitis and with determinations made by point count analysis of the relative percentage of selected components of inflammation.
