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Updated: Jul 2, 2026

Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
Experimental immunosuppression and infection of chickens with Quaranfil virus
1Department of Microbiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Abstract:
In ovo hormonal bursectomy of chick embryos was carried out by chorioallantoic membrane inoculation with testosterone propionate at the 18th day of embryonation. At hatching immunosuppression was complemented with sublethal X-irradiation. At 40 days of age the immunomanipulated and control chickens were inoculated subcutaneously with a single dose of Quaranfil virus. It was observed that bursectomy and bursectomy with sublethal X-irradiation prolonged viraemia up to the 20th day following virus infection with a significant reduction of natural haemagglutinins and of virus induced specific antibody response.

