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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
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Surface plasmon resonance protein sensor using Vroman effect.

Seokheun Choi1, Yongmo Yang, Junseok Chae

  • 1Electrical Engineering Department, Arizona State University, Tempe, AZ 85287, USA.

Biosensors & Bioelectronics
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

A novel surface plasmon resonance (SPR) sensor leverages the Vroman effect for highly sensitive and selective protein detection. This innovative approach bypasses traditional bio-receptors, enabling real-time analysis based on protein molecular weight.

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
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Published on: November 29, 2014

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Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Surface Science

Background:

  • Traditional protein detection methods often rely on complex and time-consuming bio-receptor attachment.
  • The Vroman effect describes the competitive adsorption of proteins onto surfaces, influenced by molecular weight.
  • Surface Plasmon Resonance (SPR) offers high sensitivity for detecting biomolecular interactions.

Purpose of the Study:

  • To develop a novel SPR-based protein sensor utilizing the Vroman effect for sensitive and selective protein detection.
  • To demonstrate real-time protein detection without the need for bio-receptors.
  • To evaluate the sensor's performance in distinguishing proteins of varying molecular weights.

Main Methods:

  • Microfabrication of an SPR sensor.
  • Utilizing the Vroman effect for selective protein adsorption based on molecular weight.
  • Real-time monitoring of protein adsorption and displacement using SPR.
  • Surface characterization and evaluation using Atomic Force Microscopy (AFM).

Main Results:

  • The SPR sensor successfully detected and distinguished between four different proteins (aprotinin, lysozyme, streptavidine, isolectin) based on their molecular weights.
  • The sensor demonstrated the ability to differentiate proteins with a molecular weight difference of at least 14.05kDa.
  • A very low false positive rate was achieved, highlighting the sensor's selectivity.
  • Real-time adsorption and displacement kinetics were successfully observed and analyzed.

Conclusions:

  • The developed SPR sensor effectively employs the Vroman effect for label-free, real-time protein detection.
  • This method offers a simplified and efficient alternative to traditional bio-receptor-based sensing platforms.
  • The sensor's high sensitivity, selectivity, and potential for integration with microfluidics present significant analytical capabilities.