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Updated: Jul 2, 2026

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
Published on: August 6, 2025
Pseudonocardia ailaonensis sp. nov., isolated from soil in China
Sheng Qin1, Yun-Yun Su, Yu-Qin Zhang
1The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Abstract:
A Gram-positive, aerobic actinomycete, designated strain YIM 45505(T), was isolated from a soil sample collected from Yunnan province, south-west China. Cells exhibited cream-white aerial mycelium and orange-yellow to yellow-brown substrate mycelium. The substrate mycelium fragmented into rod-shaped elements, and the aerial mycelium formed long chains of spores. 16S rRNA gene sequence analysis revealed that strain YIM 45505(T) was related most closely to Pseudonocardia oroxyli DSM 44984(T) (98.5% similarity) and Pseudonocardia halophobica IMSNU 21327(T) (97.5%). The G+C content of the DNA of strain YIM 45505(T) was 74.1 mol%. Combined with results of DNA-DNA hybridization and phenotypic tests, these data revealed that strain YIM 45505(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia ailaonensis sp. nov. is proposed. The type strain is YIM 45505(T) (=KCTC 19315(T) =DSM 44979(T)).
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