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Direct PCR of intact bacteria (colony PCR)

Michael E Woodman1

  • 1Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA.

Current Protocols in Microbiology
|September 5, 2008
PubMed

Abstract:

This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.

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