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Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...

Genital Herpes

Genital Herpes

Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...

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Related Experiment Video

Updated: Jul 2, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Published on: April 2, 2012

Herpes simplex virus: propagation, quantification, and storage.

John A Blaho¹, Elise R Morton, Jamie C Yedowitz

¹Mount Sinai School of Medicine, New York, New York, USA.

Current Protocols in Microbiology

|September 5, 2008

Summary

This summary is machine-generated.

This study details methods for propagating and quantifying herpes simplex virus (HSV), a DNA virus causing human illness. These techniques aid in diagnosing HSV infections and support research and clinical microbiology.

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Last Updated: Jul 2, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Published on: April 2, 2012

Plaquing of Herpes Simplex Viruses

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

Area of Science:

Virology
Molecular Biology
Cell Biology

Background:

Herpes simplex virus (HSV) is a significant human pathogen belonging to the Herpesviridae family.
HSV replication causes distinct cellular changes, enabling diagnostic approaches.
Understanding viral-host interactions is crucial for managing HSV infections.

Purpose of the Study:

To describe methods for propagating, quantifying, and storing HSV.
To provide techniques for diagnosing HSV infections through viral-host interactions.
To support basic research and clinical microbiology of HSV.

Main Methods:

Propagation and storage of HSV in cell culture.
Quantification of HSV stocks using infectious center assays.
Detection of viral polypeptides via immunological methods (whole cell extracts and indirect immunofluorescence).

Main Results:

Established protocols for reliable HSV propagation and storage.
Demonstrated infectious center assays for accurate HSV titration.
Validated immunological techniques for detecting HSV protein accumulation in infected cells.

Conclusions:

The described methods facilitate the study and characterization of HSV.
These techniques are valuable for both basic virology research and clinical HSV diagnostics.
Standardized protocols enhance the reliability of HSV research and patient sample analysis.