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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Laboratory maintenance of Brucella abortus
Yao-Hui Sun1, Andreas B den Hartigh, Renée M Tsolis
1University of California at Davis, Davis, California, USA.
Current Protocols in Microbiology
|September 5, 2008
Summary
This guide details culturing and storing Brucella abortus, focusing on preventing contamination and variant outgrowth. Strict Biosafety Level 3 containment and U.S. Select Agent registration are mandatory for handling this bacterium.
Area of Science:
- Microbiology
- Bacteriology
Background:
- Brucella abortus is a slow-growing bacterium.
- Effective culture and storage protocols are essential for laboratory work.
Purpose of the Study:
- To provide protocols for culturing Brucella abortus.
- To outline methods for long-term storage of Brucella abortus stocks.
- To highlight key challenges in bacterial culture and preservation.
Main Methods:
- Cultivation on solid and liquid media.
- Long-term storage techniques for bacterial stocks.
- Emphasis on preventing contamination and outgrowth of attenuated variants.
Main Results:
- Established protocols for Brucella abortus growth.
- Identified critical factors for successful long-term storage.
- Highlighted common issues like contamination and variant loss.
Conclusions:
- Standardized methods ensure reliable Brucella abortus cultures.
- Awareness of contamination and variant outgrowth is crucial.
- Compliance with Biosafety Level 3 and Select Agent regulations is mandatory.
