Related Experiment Video
Updated: Jul 2, 2026

10:37
Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
Published on: November 25, 2022
Laboratory maintenance of Borrelia burgdorferi
1University of Kansas Medical Center, Kansas City, Kansas, USA.
Current Protocols in Microbiology
|September 5, 2008
Abstract:
This unit describes the propagation of Borrelia burgdorferi as well as other "cultivable" Borrelia species in Barbour-Stoenner-Kelly-II (BSK-II) medium. It supplies a detailed recipe for BSK-II, as well as protocols for standard liquid culture, the generation of frozen Borrelia stocks, and the plating of B. burgdorferi cells in solid BSK-II medium.

