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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Commonly used fusion techniques — electroporation, polyethylene glycol...

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Antibody Structure

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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Related Experiment Video

Updated: Jul 2, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Production of polyclonal antisera.

Helen M Cooper¹, Yvonne Paterson

¹Queensland Brain Institute and University of Queensland, Brisbane, Australia.

Current Protocols in Cytometry

|September 5, 2008

Summary

This study details protocols for producing specific polyclonal antibodies in animals like rabbits and mice. These antibodies are crucial for various biological research techniques and protein analysis.

Area of Science:

Immunology
Biochemistry
Molecular Biology

Background:

Specific antibodies are essential for key techniques like immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting.
The demand for specific antibodies is increasing due to advancements in antibody-based proteomics for protein expression and modification analysis.

Purpose of the Study:

To provide protocols for generating polyclonal antisera specific for protein antigens.
To outline methods for antibody production in common laboratory animals.

Main Methods:

Immunization of animals (rabbits, rats, mice, hamsters) with purified protein or peptide antigens.
Collection and processing of antisera to yield specific polyclonal antibodies.

Main Results:

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Last Updated: Jul 2, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

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Published on: January 2, 2017

Generation of Monoclonal Antibodies Against Natural Products

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Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Published on: February 6, 2018

Established protocols for the production of specific polyclonal antibodies in multiple animal models.
Demonstrated the value of these antibodies for various biochemical and biological applications.

Conclusions:

The described protocols facilitate the timely production of essential polyclonal antibodies for research.
These methods support basic and applied research programs relying on specific antibody reagents.