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Related Experiment Video

Updated: Jul 2, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Time-resolved fluorescence measurements.

J A Steinkamp¹

¹Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

This review details time-resolved fluorescence principles using flow cytometry. It highlights advantages like discriminating fluorochromes by lifetime for applications such as bound versus free molecule determination.

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Area of Science:

Biophysics
Analytical Chemistry
Biotechnology

Background:

Time-resolved fluorescence offers advantages over traditional fluorescence spectroscopy.
Flow cytometry is a powerful technique for single-cell analysis.

Purpose of the Study:

To provide a comprehensive review of time-resolved fluorescence principles.
To demonstrate the integration of time-resolved fluorescence with flow cytometry.
To explore applications of fluorescence lifetime measurements.

Main Methods:

Detailed theoretical and practical discussion of time-resolved fluorescence measurement.
Application of time-resolved fluorescence principles within a flow cytometry context.
Compilation of common fluorescent dyes with comparative lifetime and excitation data.

Main Results:

Discrimination between fluorochromes based on fluorescence lifetime, not just emission wavelength.
Effective determination of bound versus free molecules using fluorescence lifetime.
Practical guidance on implementing time-resolved fluorescence in flow cytometry.

Conclusions:

Time-resolved fluorescence coupled with flow cytometry provides enhanced analytical capabilities.
Fluorescence lifetime measurements offer unique advantages for molecular binding studies.
This approach expands the utility of flow cytometry for complex biological analyses.