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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 2, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Plug flow cytometry.

B S Edwards¹, L A Sklar

¹University of New Mexico, Albuquerque, New Mexico, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

High-throughput flow cytometry enables rapid screening of cell collections. A new flow injection analysis system automates sample handling for large-scale biological investigations and drug discovery.

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Last Updated: Jul 2, 2026

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Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Flow cytometry is powerful for cell screening but inefficient for large-scale, multi-sample operations.
Modern drug discovery requires high-throughput screening of cellular targets against vast compound libraries.

Purpose of the Study:

To develop an automated sample handling method for high-throughput flow cytometry.
To enable efficient large-scale screening of discrete cell suspensions.

Main Methods:

Developed a flow injection analysis (FIA) approach for automated sample handling.
Implemented a plug flow system where discrete sample suspensions are injected into a carrier fluid.
Samples are sequentially delivered to the laser interrogation point of the flow cytometer.

Main Results:

The plug flow system successfully automates sample delivery for flow cytometry.
Precisely defined volumes of individual sample suspensions are sequentially processed.
Demonstrated the system's capability for high-throughput analysis.

Conclusions:

The developed flow injection analysis plug flow system enhances the efficiency of flow cytometry for large-scale screening.
This automated approach is beneficial for applications like drug discovery and broader biological investigations.
The system provides a foundation for advancing high-throughput cellular analysis.