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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Related Experiment Video

Updated: Jul 2, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
13:44

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

High-speed cell sorting.

J F Leary1

  • 1University of Texas Medical Branch, Galveston, Texas, USA.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

Flow cytometry rapidly analyzes cells for rare event detection. Cell sorting isolates specific cells based on multiple properties, optimizing speed and resolution for biological and clinical applications.

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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Related Experiment Videos

Last Updated: Jul 2, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
13:44

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
15:29

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Flow cytometry analyzes thousands of cells per second, ideal for rare event detection.
  • Isolation of cells based on multiple quantitative properties is crucial for biological and clinical applications.
  • Flow cytometry's analytical power enables cell sorting for physical isolation.

Purpose of the Study:

  • To discuss the principles and practices of operating a cell sorter.
  • To maximize the sorting rate of a cell sorter.
  • To explore the trade-offs between speed and resolution in cell sorting.

Main Methods:

  • Principles of flow cytometry operation.
  • Practices for optimizing cell sorter performance.
  • Analysis of speed versus resolution in cell sorting.

Main Results:

  • Flow cytometry enables high-throughput cell analysis.
  • Cell sorting provides real-time data classification and physical isolation.
  • Maximizing sorting rate involves managing speed-resolution trade-offs.

Conclusions:

  • Cell sorting, powered by flow cytometry, is essential for isolating specific cell populations.
  • Optimizing cell sorter operation balances sorting speed with the desired resolution.
  • Understanding these principles is key for effective cell isolation in research and clinical settings.