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Related Concept Videos

Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
Non-gated Ion Channels01:24

Non-gated Ion Channels

Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Non-gated Ion Channels01:24

Non-gated Ion Channels

Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

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P50 Sensory Gating in Infants
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P50 Sensory Gating in Infants

Published on: December 26, 2013

Principles of gating.

J C Wood1

  • 1Coulter Corporation, Miami, Florida, USA.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

This guide explains flow cytometry gating principles, a fundamental technique for all applications. It details how gating works, its applications, and compares various methods for optimal data analysis.

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Area of Science:

  • * Biotechnology and Immunology
  • * Cellular Analysis Technologies

Background:

  • * Flow cytometry is a powerful technique for analyzing cellular characteristics.
  • * Gating is a critical, yet often complex, aspect of flow cytometry data analysis.
  • * Understanding gating principles is essential for accurate interpretation of results.

Purpose of the Study:

  • * To provide a comprehensive overview of flow cytometry gating principles.
  • * To explain the conceptual framework and practical application of gating techniques.
  • * To compare the advantages and disadvantages of different gating strategies.

Main Methods:

  • * Conceptual review of gating principles in flow cytometry.
  • * Analysis of historical development in gating understanding.
  • * Detailed examination of various gating techniques and their applicability.

Main Results:

  • * Gating is a universal principle across all flow cytometry applications.
  • * Provides clear guidance on appropriate and inappropriate use of gating techniques.
  • * Highlights the benefits and drawbacks of diverse gating methodologies.

Conclusions:

  • * This resource is essential for flow cytometry technicians and new graduate students.
  • * Offers a valuable refresher for experienced researchers in the field.
  • * Ensures high technical accuracy and historical context in understanding gating.