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Updated: Jul 2, 2026

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Published on: January 29, 2010

Titering antibodies.

C C Stewart¹, S J Stewart

¹Roswell Park Cancer Institute, Buffalo, New York, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Determining the optimal antibody concentration is crucial for accurate automated cellular identification using flow cytometry. This study outlines methods for precise antibody titration to ensure reliable immunophenotyping results in routine laboratory use.

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Determining the Phagocytic Activity of Clinical Antibody Samples

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Published on: November 30, 2011

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Last Updated: Jul 2, 2026

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Published on: January 29, 2010

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

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Published on: September 7, 2018

Determining the Phagocytic Activity of Clinical Antibody Samples

Determining the Phagocytic Activity of Clinical Antibody Samples

Published on: November 30, 2011

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Flow cytometry is a key technique for automated cell identification.
Accurate immunophenotyping relies on optimal antibody concentrations.
Antibody titration is essential for reproducible flow cytometry experiments.

Purpose of the Study:

To present methods for determining optimal antibody titers for flow cytometry.
To address the critical issue of antibody concentration in immunophenotyping.
To emphasize quality control for antibody batch preparation.

Main Methods:

Techniques for titrating single, dual, and multiple antibodies.
Application of fluorochrome-conjugated antibodies.
Quality control measures for antibody preparation.

Main Results:

Established protocols for antibody titer determination.
Demonstrated importance of precise antibody amounts.
Highlighted quality control for routine antibody use.

Conclusions:

Optimal antibody titration is vital for accurate flow cytometry.
Standardized methods ensure reliable immunophenotyping.
Quality control is paramount for consistent experimental outcomes.