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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism (Microcystis aeruginosa) Using Flow Cytometry
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Cellular function probes.

I D Johnson1

  • 1Molecular Probes, Inc., Eugene, Oregon, USA.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

This unit details fluorescent probes for cell biology, including green fluorescent protein and ion probes. Understanding probe chemistry and cellular behavior is crucial for accurate fluorescence imaging and analysis.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • Fluorescent probes are essential tools in cell biology for visualizing cellular components and processes.
  • A wide array of probes exist, targeting diverse cellular molecules and ions.
  • Effective utilization necessitates understanding probe chemistry and cellular interactions.

Purpose of the Study:

  • To provide a comprehensive overview of commonly used fluorescent probes in cell biology.
  • To elucidate the chemical relationships and functional applications of these probes.
  • To emphasize the importance of understanding probe uptake, distribution, and factors affecting fluorescence.

Main Methods:

  • Review of fluorescent probe types, including protein-based (e.g., green fluorescent protein) and small-molecule probes.
  • Discussion of chemical properties and their correlation with functional applications.
  • Analysis of cellular factors influencing probe performance, such as uptake, distribution, and environmental effects on fluorescence.

Main Results:

  • Categorization of fluorescent probes based on their targets (e.g., esterase/peptidase substrates, thiols, lipids, ions).
  • Explanation of chemical principles underlying probe function and application.
  • Identification of critical factors affecting fluorescence emission, particularly for pH and calcium probes.

Conclusions:

  • Proper application of fluorescent probes requires knowledge of their chemical nature and cellular behavior.
  • Understanding probe-specific characteristics is vital for reliable experimental outcomes in cell biology.
  • This unit serves as a guide for selecting and using fluorescent probes effectively.