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Related Experiment Video

Updated: Jul 2, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Molecular combing.

C Conti¹, S Caburet, C Schurra

¹Institut Pasteur, Paris, France.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Molecular combing is a new fiber-FISH technique that gently stretches single DNA molecules for rapid, quantitative genome-wide studies. This method allows for numerous accurate measurements in a single experiment.

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MALDI-ToF MS Method for the Characterization of Synthetic Polymers with Varying Dispersity and End Groups

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Published on: October 3, 2025

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Fiber-fluorescence in situ hybridization (FISH) is a powerful technique for analyzing DNA.
Existing methods can be time-consuming and require extensive resources.

Purpose of the Study:

To describe molecular combing, an advancement in fiber-FISH technology.
To provide protocols for various molecular combing applications.

Main Methods:

Molecular combing involves binding single DNA molecules to a surface and stretching them using a receding meniscus.
The technique is gentle, rapid, and easy to perform.
Detailed protocols cover surface and DNA preparation, probe labeling, basic combing, high-resolution physical mapping, and gene-dosage analysis.

Main Results:

Molecular combing yields reliable, quantitative data for genome-wide studies.
The technique allows for a large number of accurate measurements in a single experiment.
It eliminates the need for additional complex techniques.

Conclusions:

Molecular combing represents a significant improvement in DNA analysis technology.
The provided protocols enable researchers to implement this technique for various genomic applications.