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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Updated: Jul 2, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
09:11

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Immunocytochemical detection.

J Wiegant1

  • 1Leiden University, Leiden, The Netherlands.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

This unit details direct and indirect in situ hybridization protocols for microscopy. It covers chromogenic and immunofluorescence detection methods, aiding in selecting the best strategy.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • In situ hybridization (ISH) is crucial for visualizing nucleic acid targets within cellular or tissue contexts.
  • Selecting appropriate detection methods is vital for successful ISH experiments.

Purpose of the Study:

  • To provide comprehensive protocols for both direct and indirect in situ hybridization.
  • To detail methods compatible with bright-field microscopy and immunofluorescence detection.
  • To offer guidance on choosing the optimal detection strategy.

Main Methods:

  • Detailed protocols for direct and indirect in situ hybridization.
  • Adaptations for bright-field microscopy utilizing precipitating chromogenic substrates.
  • Protocols for immunofluorescence detection employing hapten-labeled probes.

Main Results:

  • Successful implementation of both direct and indirect ISH methods.
  • Demonstration of effective chromogenic and immunofluorescence detection strategies.
  • Provision of alternative procedures to suit diverse experimental needs.

Conclusions:

  • The unit offers a robust framework for performing in situ hybridization.
  • Guidance on method selection enhances experimental efficiency and reliability.
  • These protocols support diverse applications in biological research.