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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

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Method for visualizing aerosol contamination in flow sorters.

Andrew S Oberyszyn¹

¹The Ohio State University Comprehensive Cancer Center, Analytical Cytometry Shared Resource Laboratory, Ohio State University, Columbus, Ohio, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Flow cytometers can release aerosols, posing contamination risks. A new, simple visual method using fluorescent resin effectively assesses aerosol containment, ensuring operator safety.

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Area of Science:

Biomedical Engineering
Laboratory Safety
Analytical Chemistry

Background:

Flow cytometers generate aerosols during operation.
Existing instruments often have incomplete aerosol elimination.
Aerosolized contaminants pose risks to operators and facility personnel, especially with biohazardous samples.

Purpose of the Study:

To describe a visual method for assessing aerosol containment in flow cytometers.
To provide a rapid, inexpensive, and simple technique for evaluating instrument performance.

Main Methods:

Utilized a commercial resin that fluoresces under UV or black light.
Applied the resin to visually examine aerosol containment during flow cytometer operation.
Evaluated the method's speed, cost-effectiveness, and simplicity.

Main Results:

The described method provides immediate visual feedback on aerosol containment.
The technique is rapid, inexpensive, and simple to implement.
Results are potentially quantitative, offering measurable insights into containment effectiveness.

Conclusions:

This visual method offers an effective means to assess flow cytometer aerosol containment.
The technique enhances laboratory safety by identifying potential exposure risks.
The method is a valuable tool for routine instrument validation and maintenance.