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Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Immunophenotyping using a laser scanning cytometer.

Attila Tárnok¹, Andreas O H Gerstner

¹University of Leipzig, Leipzig, Germany.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Laser scanning cytometry offers slide-based immunophenotyping, overcoming flow cytometry

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Traditional flow cytometry enables immunophenotyping and cell proliferation analysis.
Limitations include inability to analyze cell morphology and restain cells of interest.

Purpose of the Study:

To describe a slide-based immunophenotyping method using laser scanning cytometry (LSC).
To highlight the advantages of LSC over traditional flow cytometry for cell analysis.

Main Methods:

Adaptation of flow cytometry assays for LSC.
Utilizing a laser scanning cytometer for slide-based immunophenotyping.

Main Results:

LSC allows for repeated analysis and archiving of specimens.
Morphological examination and further staining provide additional data.
Cells are not lost during LSC analysis, unlike flow cytometry.

Conclusions:

LSC offers enhanced data acquisition through morphological analysis and restaining.
The slide-based method has potential as a powerful tool in clinical diagnosis.
LSC overcomes limitations of traditional flow cytometry by preserving cells and enabling repeated analysis.