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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 2, 2026

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Published on: September 18, 2020

Mycobacterium tuberculosis susceptibility testing by flow cytometry.

Ronald F Schell¹, Dean T Nardelli, David J DeCoster

¹University of Wisconsin, Madison, Wisconsin, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Tuberculosis affects one-quarter of the world population. Rapid susceptibility testing using flow cytometry provides accurate results within 24 hours, improving disease control and public health measures.

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Last Updated: Jul 2, 2026

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Published on: September 18, 2020

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

Area of Science:

Microbiology
Immunology
Public Health

Background:

Tuberculosis (TB) is a significant global health challenge, with widespread population infection.
Effective control of TB relies on timely and precise susceptibility testing of Mycobacterium tuberculosis.

Purpose of the Study:

To evaluate the efficacy of flow cytometry for rapid susceptibility testing of Mycobacterium tuberculosis.
To compare the speed and accuracy of flow cytometry with traditional methods for TB susceptibility testing.

Main Methods:

Utilized flow cytometry for susceptibility testing of tubercle bacilli.
Compared results and turnaround time against conventional diagnostic methods.

Main Results:

Flow cytometry achieved accurate susceptibility testing within twenty-four hours.
Traditional methods require days to weeks for comparable results.

Conclusions:

Flow cytometry offers a significantly faster and accurate method for TB susceptibility testing.
This advancement can enhance public health strategies for TB prevention and control.