Related Experiment Video
Updated: Jul 2, 2026

Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Principles and applications of PRINS in cytogenetics
1Laboratory of Molecular Pathology, Institute of Pathology, Aarhus Kommunehospital, Aarhus, Denmark.
Abstract:
A flexible, low-cost alternative to FISH, primed in situ labeling (PRINS) has traditionally been used to detect tandemly repeated target sequences in chromosomes and nuclei. The technique is capable of discriminating among closely related DNA sequences in situ and has the advantage of using very small probes which easily penetrate to almost any target. This unit describes basic PRINS and the alternative version, dideoxy-PRINS, which can increase the sensitivity of the reaction by an order of magnitude. New material on multicolor PRINS and quantitative PRINS has been added. Protocols for detection of single-copy sequences and for application to the study of in-vivo activity of DNA-modifying enzymes are planned.
Related Concept Videos
Karyotyping
Karyotyping
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Chromosomal Theory of Inheritance
Polytene Chromosomes
Hardy-Weinberg Principle

