Related Experiment Video
Updated: Mar 30, 2026
11:47
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
Published on: March 4, 2022
2.7K
Live-animal imaging of renal function by multiphoton microscopy
Kenneth W Dunn1, Timothy A Sutton, Ruben M Sandoval
1Indiana University School of Medicine, Indianapolis, Indiana, USA.
Current Protocols in Cytometry
|September 5, 2008
Summary
Intravital microscopy offers high-resolution imaging of living kidneys, revealing cellular structures and functions. This advanced technique analyzes kidney processes like permeability and blood flow in real-time within intact animals.
Area of Science:
- Physiology
- Microscopy
- Renal Biology
Background:
- Intravital microscopy combines cellular resolution with in vivo relevance.
- Recent advancements include multiphoton fluorescence microscopy for deep tissue imaging.
- This technique is crucial for studying the intact kidney's structure and function.
Purpose of the Study:
- To detail the application of multiphoton intravital microscopy for high-resolution kidney research.
- To highlight the capability of imaging glomeruli, tubules, and vasculature in living kidneys.
- To present a method for evaluating various kidney functions in vivo.
Main Methods:
- Utilizing multiphoton fluorescence microscopy for deep kidney imaging.
- Administering fluorescent probes to anesthetized, surgically prepared animals.
- Acquiring images for up to 3 hours with subsequent digital image analysis.
Main Results:
- Subcellular resolution achieved deep within the living kidney.
- High-resolution characterization of kidney structures (glomeruli, tubules, vasculature).
- Demonstrated potential for evaluating diverse kidney functions.
Conclusions:
- Multiphoton intravital microscopy is a powerful tool for in vivo kidney research.
- This technique enables detailed analysis of kidney structure and dynamic processes.
- It supports the evaluation of glomerular permeability, tubular function, and vascular dynamics.
More Related Videos
Related Concept Videos
01:19Imaging Studies VII: Vascular Imaging
470
DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
470
01:24Imaging Studies II: Ultrasonography
723
IntroductionUltrasonography, or renal ultrasound, is a noninvasive medical imaging technique that uses high-frequency sound waves to visualize the kidneys, ureters, bladder, and surrounding tissues.Indications for Urinary System UltrasonographyUrinary system ultrasonography is indicated in various clinical scenarios, such as:Kidney Stones (Urolithiasis): To detect and monitor the size and presence of kidney or urinary tract stones.Hydronephrosis: To assess the dilation of the renal pelvis and...
723
01:28Imaging Studies I: Kidney, Ureter, and Bladder Studies
646
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
646