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Updated: Jul 2, 2026

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

High-throughput cytotoxicity screening by propidium iodide staining.

Bruce S Edwards¹, Irena Ivnitski-Steele, Susan M Young

¹University of New Mexico, Albuquerque, New Mexico, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

This automated system rapidly analyzes cell cytotoxicity using flow cytometry and propidium iodide staining in microplates. It enables high-throughput screening with minimal sample and reagent use.

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Last Updated: Jul 2, 2026

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Published on: January 7, 2019

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Area of Science:

Biomedical Engineering
Cell Biology
Assay Development

Background:

High-throughput screening is crucial for drug discovery and toxicology.
Existing methods for cell cytotoxicity analysis can be time-consuming and require large sample volumes.

Purpose of the Study:

To describe an automated system for high-throughput cell cytotoxicity analysis.
To enable rapid quantification of cytotoxicity using minimal sample volumes.

Main Methods:

Automated analysis of cell cultures in 96-well and 384-well microplates.
Utilizes flow cytometry for cell counting and propidium iodide fluorescence analysis.
Aspiration of only 2 microliters per sample for analysis.

Main Results:

Achieves analysis rates of 40 samples per minute.
Enables rapid analysis of 96-well plates in approximately 2.5 minutes and 384-well plates in approximately 10 minutes.
Quantifies cytotoxicity based on combined cell counting and propidium iodide uptake.

Conclusions:

The described system offers an efficient and cost-effective solution for high-throughput cell cytotoxicity assessment.
This automated approach minimizes reagent consumption and sample volume, making it suitable for large-scale screening.