Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-Dependent Layer Formation Process on Quartz Bed Particles during the Fast Pyrolysis Process of Wood.

ACS omega·2026
Same author

Bed Particle Layer Formation and Characteristics of Ilmenite Bed Particles Utilized in Fluidized Bed Combustion of Chicken Litter.

ACS omega·2025
Same author

Thermodynamic Equilibrium Study of Ash Transformation during Entrained Flow Conversion of Agricultural Biomass Focusing on the Potential Extraction of Valuable Si and K-P Compounds via Condensation from the Gas Phase.

ACS omega·2025
Same author

Study of the aberrant retention behavior of a semi-crystalline polyamide in reversed-phase liquid chromatography.

Journal of chromatography. A·2025
Same author

Size-Exclusion Chromatography-Electrospray-Ionization Mass Spectrometry To Characterize End Group and Chemical Distribution of Poly(lactide-<i>co</i>-glycolide) Copolymers.

Journal of the American Society for Mass Spectrometry·2025
Same author

Four-Dimensional Lipidomic Analysis Using Comprehensive Online UHPLC × UHPSFC/Tandem Mass Spectrometry.

Analytical chemistry·2024
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
Same journal

Stereo-sensitive modelling of gas chromatographic retention indices of mono-cycloalkanes in jet fuel range.

Journal of chromatography. A·2026
Same journal

Approaches to using retention indices with coupled column pressure tuning in gas chromatography.

Journal of chromatography. A·2026
Same journal

MOF-supported surface-imprinted polymer for hazard governance of aristolochic acids in herbal matrices: A safety-control strategy supported by multiscale simulations.

Journal of chromatography. A·2026
See all related articles

Related Experiment Video

Updated: Jul 2, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
14:53

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Methacrylate monolithic capillary columns for gradient peptide separations.

Peter Pruim1, Marcus Ohman, Yuli Huo

  • 1Polymer-Analysis Group, Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.

Journal of Chromatography. A
|September 6, 2008
PubMed
Summary
This summary is machine-generated.

Poly(butyl methacrylate-co-ethylene dimethacrylate) (BMA) monolithic columns offer good peptide separation in steep gradients but show lower peak capacity in shallow gradients compared to packed columns. Modifying methacrylate columns with lauryl methacrylate (LMA) or using longer BMA columns improves performance.

More Related Videos

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
10:27

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Related Experiment Videos

Last Updated: Jul 2, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
14:53

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
10:27

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Monolithic capillary columns offer potential advantages in liquid chromatography.
  • Evaluating their performance against traditional packed columns is crucial for method development.

Purpose of the Study:

  • To assess the performance of a poly(butyl methacrylate-co-ethylene dimethacrylate) (BMA) monolithic capillary column for peptide separation using gradient-elution liquid chromatography.
  • To compare its peak capacity with a conventional C18-modified silica packed column.

Main Methods:

  • Preparation and testing of a BMA monolithic capillary column.
  • Comparison of peptide retention and peak capacity against a C18-packed capillary column.
  • Analysis of peak width and retention factor relationships under various gradient conditions.

Main Results:

  • BMA columns exhibited lower peptide retention than C18 columns, requiring lower acetonitrile concentrations.
  • Peak capacity of BMA columns was significantly lower than packed columns in shallow gradients (approx. 90 vs. 150).
  • BMA columns performed comparably to packed columns in steep gradients (approx. 75 peak capacity).
  • Using lauryl methacrylate (LMA) or longer BMA columns improved peak capacity (approx. 125-135).

Conclusions:

  • Methacrylate monolithic columns show promise for peptide separation, with performance dependent on gradient steepness.
  • Strategies like LMA modification or column lengthening can enhance peak capacity for monolithic columns.
  • BMA monolithic columns are suitable for applications requiring steep gradients, while LMA or longer BMA columns offer broader applicability.