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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
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Published on: October 31, 2015

Analog single-photon counter for high-speed scanning microscopy.

Sucbei Moon1, Dug Young Kim

  • 1Department of Information and Communications, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea. moonsb@gist.ac.kr

Optics Express
|September 6, 2008
PubMed
Summary

We developed a new analog single-photon counting (SPC) method for high-speed scanning microscopy. This technique improves measurement speed and dynamic range, offering stable, sensitive photodetection with reduced noise for better image quality.

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Area of Science:

  • Photonics
  • Microscopy
  • Electronic Engineering

Background:

  • High-speed scanning microscopy demands photodetectors with high measurement speed and wide dynamic range.
  • Traditional methods face limitations in speed and noise, particularly from photomultiplier tube (PMT) afterpulsing.

Purpose of the Study:

  • To introduce a novel analog single-photon counting (SPC) method for enhanced photodetection in high-speed scanning microscopy.
  • To improve measurement speed, dynamic range, and image quality by addressing PMT afterpulsing noise.

Main Methods:

  • Implementation of an analog SPC scheme using electronic circuits for signal differentiation and integration.
  • Utilizing analog circuitry for signal processing prior to and following signal discrimination from a PMT.

Main Results:

  • The analog SPC scheme demonstrates high sensitivity and operational stability with a simpler implementation.
  • Achieved significantly improved maximum count rates due to fast analog circuitry operation.
  • Successfully neutralized impulse noises from PMT afterpulsing, resulting in nearly shot-noise-limited imaging performance.

Conclusions:

  • The developed analog SPC method is highly suitable for high-speed scanning microscopy applications.
  • The scheme offers a robust solution for achieving high-speed, wide dynamic range photodetection with superior image quality.