Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Deactivation Processes: Jablonski Diagram01:25

Deactivation Processes: Jablonski Diagram

Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The energy-level diagram, or Jablonski diagram, is a graphical representation of these interactions, illustrating the various states and transitions a molecule can undergo. In a typical Jablonski diagram, the lowest horizontal line represents the ground-state energy of the molecule, which is usually a singlet state. This state represents the energies...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Actin Polymerization01:42

Actin Polymerization

Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight actin...
Actin Filament Depolymerization01:19

Actin Filament Depolymerization

Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
Cycloaddition Reactions: MO Requirements for Photochemical Activation01:12

Cycloaddition Reactions: MO Requirements for Photochemical Activation

Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interferometric scattering for optical tomoslicing of transparent solids.

Light, science & applications·2026
Same author

Hyperspectral Imaging of Dipole-Ladder-Mediated Exciton Drift in Moiré Superlattices.

ACS nano·2026
Same author

Free-Space Skyrmions Radiated from a Geometric Phase Aperture.

ACS nano·2026
Same author

Helium as an exceptional protective gas for femtosecond laser processing of crystalline silicon.

Optics express·2026
Same author

Theoretical study on the effects of ligand on the ligand-protected gold nanoclusters.

Journal of molecular graphics & modelling·2026
Same author

Superadiabatic topological pumping on photonic chips.

Nature communications·2025

Related Experiment Video

Updated: Jul 2, 2026

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

Two-photon induced polymer nanomovement.

Hidekazu Ishitobi1, Satoru Shoji, Tsunemi Hiramatsu

  • 1Nanophotonics Laboratory, RIKEN The Institute of Physical and Chemical Research, Wako, Saitama, Japan

Optics Express
|September 6, 2008
PubMed
Summary
This summary is machine-generated.

This study demonstrates two-photon induced plastic surface deformation in azo polymer films. Intense laser irradiation causes nanomovement via photoselective isomerization, dependent on light polarization and wavelength.

More Related Videos

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Related Experiment Videos

Last Updated: Jul 2, 2026

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Area of Science:

  • Polymer Science
  • Optics
  • Materials Science

Background:

  • Azo polymers exhibit photoresponsive behavior due to trans-cis isomerization.
  • Surface deformation in polymers can be induced by light, but two-photon effects are less explored.

Purpose of the Study:

  • To report the first instance of two-photon induced plastic surface deformation in solid polymer films.
  • To investigate the mechanism of photofluidic polymer nanomovement using intense laser irradiation.

Main Methods:

  • Exposure of azo polymer films to intense 920 nm laser irradiation.
  • Analysis of polarization-dependent and wavelength-dependent surface deformations.
  • Investigation of light intensity gradients and spot positioning effects.

Main Results:

  • Observed plastic surface deformation in azo polymer films.
  • Demonstrated photofluidic polymer nanomovement driven by two-photon trans-cis isomerization.
  • Deformation is dependent on laser wavelength, polarization, and light intensity gradients.

Conclusions:

  • Two-photon absorption is a viable mechanism for inducing plastic deformation in azo polymers.
  • Photoselective isomerization under intense irradiation leads to controlled polymer surface modification.
  • Precise control over nanomovement is achievable by manipulating laser parameters.