Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oncocytic papillary cystadenoma.

British dental journal·2024
Same author

Cell-free DNA Testing in Lung Cancer Patients Improves National Health Service Real-world Result Turnaround Time and Treatment Initiation for Tumours with Targetable Alterations by 4 Weeks.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023
Same author

Adaptations of an Effective Evidence-Based Pediatric Weight Management Intervention.

Prevention science : the official journal of the Society for Prevention Research·2023
Same author

Intracellular Antibodies for Drug Discovery and as Drugs of the Future.

Antibodies (Basel, Switzerland)·2023
Same author

IL-1 Signal Inhibition In Alcoholic Hepatitis (ISAIAH): a study protocol for a multicentre, randomised, placebo-controlled trial to explore the potential benefits of canakinumab in the treatment of alcoholic hepatitis.

Trials·2021
Same author

Evaluation of intranasal nafamostat or camostat for SARS-CoV-2 chemoprophylaxis in Syrian golden hamsters.

bioRxiv : the preprint server for biology·2021

Related Experiment Video

Updated: Jul 2, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Microdissection molecular copy-number counting (microMCC)--unlocking cancer archives with digital PCR.

F McCaughan1, E Darai-Ramqvist, A T Bankier

  • 1Centre for Respiratory Research, Department of Medicine, Royal Free and University College Medical School, The Rayne Institute, London WC1E 6JJ, UK.

The Journal of Pathology
|September 6, 2008
PubMed
Summary

Micro molecular copy-number counting (microMCC) precisely measures DNA copy number variations in degraded clinical samples. This digital PCR technique enables high-resolution cancer genome analysis from archival biopsies.

More Related Videos

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
09:16

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

Related Experiment Videos

Last Updated: Jul 2, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
09:16

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • Cancer genomes exhibit copy number variations (CNVs) like deletions and amplifications, crucial for tumor development and patient prognosis.
  • Existing methods for CNV analysis are limited by sample quality, dynamic range, and scalability, hindering research on archived clinical samples.
  • Molecular copy-number counting (MCC) is a digital PCR technique previously used for CNV analysis in high-quality DNA.

Purpose of the Study:

  • To adapt MCC for analyzing degraded DNA from formalin-fixed paraffin-embedded (FFPE) clinical biopsies.
  • To demonstrate the capability of microMCC to precisely assess copy number variation across multiple genomic loci.
  • To showcase the application of microMCC in high-resolution cancer genome interrogation, particularly at amplified segment junctions.

Main Methods:

  • Development of micro molecular copy-number counting (microMCC), an adaptation of digital PCR.
  • Application of microMCC to DNA extracted from FFPE clinical biopsies, including laser capture microdissected samples.
  • Analysis of picogram quantities of degraded DNA to assess copy number variation at multiple loci.

Main Results:

  • MicroMCC accurately assesses copy number variation over a significant dynamic range using DNA from FFPE biopsies.
  • The technique precisely measures CNVs at multiple loci even with picogram quantities of degraded DNA.
  • MicroMCC successfully defined the junction between amplified and non-amplified genomic segments in a bronchial carcinoma sample.

Conclusions:

  • MicroMCC is a powerful tool for high-resolution targeted cancer genomics using archived clinical specimens.
  • This method overcomes limitations of conventional techniques, enabling analysis of small and degraded DNA samples.
  • MicroMCC holds significant potential for exploiting archival resources and advancing cancer diagnostics and prognostics.