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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
PtdIns5P regulation through evolution: roles in membrane trafficking?
Odile Lecompte1, Olivier Poch, Jocelyn Laporte
1Department of Structural Biology and Genomics, rue Laurent Fries, Illkirch, F-67400 France.
Abstract:
Phosphoinositides are lipid second messengers that are essential for many cellular processes, including signal transduction and cell compartmentalization. Among them, phosphatidylinositol 5-phosphate (PtdIns5P) is the least characterized, although several proteins involved in its regulation are implicated in human diseases. We studied the distribution of 32 PtdIns5P-metabolizing proteins in 39 eukaryotic genomes. Phylogenetic profiles identify four groups of co-evolving proteins, confirming known protein complexes and revealing new ones. The complexes comprise a phosphatase, a kinase and a regulator; this indicates that physical interactions between the three partners are necessary for the acute spatial regulation of PtdIns5P turnover. By examining PtdIns5P metabolism in this new perspective, we propose a role for PtdIns5P in membrane trafficking from late endosomal compartments to the plasma membrane.
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