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Related Experiment Video

Updated: Jul 1, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
13:29

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

Gram-negative bloodstream infections.

Patricia Muñoz1, Ana Fernandez Cruz, Marta Rodríguez-Créixems

  • 1Microbiology and Infectious Disease Department, Hospital General Universitario Gregorio Marañón, CIBER de Enfermedades Respiratorias, Universidad Complutense, Madrid, Spain. pmunoz@micro.hggm.es

International Journal of Antimicrobial Agents
|September 9, 2008
PubMed
Summary
This summary is machine-generated.

This study tracked bloodstream infections (BSIs) over 22 years in a large hospital. It details the incidence of BSIs and analyzes the Gram-negative organisms causing them.

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Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Epidemiology

Background:

  • Prospective data collection on bloodstream infections (BSIs) over 22 years at a tertiary teaching hospital.
  • The study encompasses a large patient population (650,000-750,000) and significant hospital bed capacity (1750-2500 beds).
  • Standardized definitions and microbiological methods were employed throughout the study period.

Purpose of the Study:

  • To analyze the microbiological workload of blood cultures.
  • To report the evolution of bloodstream infection incidence over 22 years.
  • To describe Gram-negative organisms recovered from blood cultures and discuss their clinical significance.

Main Methods:

  • Prospective data recording of bloodstream infections (BSIs).
  • Analysis of blood culture microbiological workload and incidence rates (per 1000 admissions and per 100,000 inhabitants).
  • Identification and characterization of Gram-negative organisms from blood cultures.

Main Results:

  • Over one million admissions during the study period.
  • Significant increase in blood culturing from 299/1000 admissions in 1985 to 720/1000 admissions in 2006.
  • 65,475 blood cultures yielded significant microorganisms, leading to 27,419 episodes of significant BSI in 22,626 patients.

Conclusions:

  • The study provides a comprehensive 22-year overview of bloodstream infections in a major hospital setting.
  • Analysis highlights trends in BSI incidence and microbiological findings.
  • Detailed description of Gram-negative organisms and their clinical relevance in BSIs is presented.