Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CRISPR-Cas9-mediated upregulation of utrophin ameliorates Duchenne muscular dystrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Transient versican expression is required for β1-integrin accumulation during podocyte layer morphogenesis in amphibian developing kidney.

Cells & development·2025
Same author

Retinal organoids mirror CRISPR-Cas9 gene editing efficiency observed <i>in vivo</i>.

Molecular therapy. Methods & clinical development·2025
Same author

Comprehensive analysis of Ephrin ligand and receptor expression reveals exclusive domains during nephrogenesis for epha4/epha7 and efna3.

The International journal of developmental biology·2025
Same author

Eps2Fold: a rapid method to characterize G-quadruplex DNA structures using single absorbance spectra.

Nucleic acids research·2025
Same author

Direct delivery of Cas9 or base editor protein and guide RNA complex enables genome editing in the retina.

Molecular therapy. Nucleic acids·2024

Related Experiment Video

Updated: Jul 1, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

DNA and RNA quadruplex ligands.

Anne De Cian1, Julien Gros, Aurore Guédin

  • 1Muséum national d'Histoire naturelle USM 503, INSERM U565, CNRS UMR5153, Paris, France.

Nucleic Acids Symposium Series (2004)
|September 9, 2008
PubMed
Summary
This summary is machine-generated.

Guanine quadruplexes (G4) are unique nucleic acid structures. This study investigates how a specific molecule binds to both RNA and DNA G4 structures, revealing insights into their stability and recognition.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Related Experiment Videos

Last Updated: Jul 1, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Guanine-rich nucleic acids form complex structures known as guanine quadruplexes (G4).
  • Both RNA and DNA can form these G4 structures, influencing their biological roles.
  • Understanding G4 stability and ligand interactions is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the binding interactions of a pyridine dicarboxamide derivative with G4 structures.
  • To explore the rules governing the stability and specificity of RNA and DNA quadruplexes.
  • To compare the binding affinities of the ligand to various oligoribo- and oligodeoxyribo-nucleotides.

Main Methods:

  • Synthesis and characterization of pyridine dicarboxamide derivative.
  • Preparation of various oligoribo- and oligodeoxyribo-nucleotides.
  • Binding assays to determine ligand interaction with different G4 structures.

Main Results:

  • The pyridine dicarboxamide derivative exhibits binding to both RNA and DNA G4 structures.
  • Differential binding affinities were observed between RNA and DNA quadruplexes.
  • Structural analysis provided insights into the molecular basis of ligand recognition.

Conclusions:

  • The pyridine dicarboxamide derivative is a potential ligand for targeting G4 structures.
  • The study highlights key differences in ligand binding between RNA and DNA G4s.
  • Findings contribute to the understanding of G4-ligand interactions for potential therapeutic applications.