Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.6K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.4K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visualizing cellular G-quadruplexes with a bioinspired chemiluminescent probe.

Nucleic acids research·2026
Same author

The impact of chemical modifications of antisense oligonucleotides on CTX-M-15 β-lactamase target affinity and expression of ceftriaxone-resistant <i>Escherichia coli</i>.

NAR molecular medicine·2026
Same author

High-throughput measurement and prediction of the i-motif DNA stability landscape.

Nucleic acids research·2026
Same author

Archaeal G-quadruplexes: a novel model for understanding unusual DNA/RNA structures across the tree of life.

Nucleic acids research·2026
Same author

Pentanucleotide guanine-rich WGGGW repeats, including CANVAS AGGGA repeats, form a variety of noncanonical structures.

Nucleic acids research·2026
Same author

G-quadruplex structures as modulators of alternative promoter usage.

NAR genomics and bioinformatics·2026

Related Experiment Video

Updated: Jan 16, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.6K

Eps2Fold: a rapid method to characterize G-quadruplex DNA structures using single absorbance spectra.

Eric Largy1, Aurore Guédin1, Amani Kabbara1

  • 1Univ. Bordeaux, INSERM, U1212, CNRS, UMR 5320, ARNA, IECB, Bordeaux F-33000, France.

Nucleic Acids Research
|September 29, 2025
PubMed
Summary
This summary is machine-generated.

Eps2Fold is a new method that uses UV absorbance spectra to quickly and affordably characterize G-quadruplex (G4) structures. This technique offers high structural resolution, similar to circular dichroism, aiding G4 research.

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.8K

Related Experiment Videos

Last Updated: Jan 16, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.6K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • G-rich oligonucleotides form G-quadruplexes (G4s), polymorphic structures crucial in biological processes and nanotechnology.
  • Understanding G4 formation requires simple, rapid detection and characterization assays.

Purpose of the Study:

  • To introduce Eps2Fold, a novel method for detecting and characterizing G-quadruplex conformations using a single UV-absorbance spectrum.
  • To demonstrate the efficacy of Eps2Fold in providing high structural resolution comparable to circular dichroism.

Main Methods:

  • Eps2Fold subtracts an experimentally recorded UV spectrum of a folded G4 from a calculated spectrum of its unfolded state.
  • Comprehensive biophysical and principal component analysis (PCA) were performed on thirty G4-forming oligonucleotides.
  • An open-source GUI program was developed for automated spectral analysis via a web application.

Main Results:

  • Eps2Fold generates spectral signatures with high structural resolution, similar to circular dichroism (CD) spectra.
  • The method allows precise G4 topology assignment based on stacked guanine glycosidic bond angles.
  • Multivariate statistical approaches like PCA are essential for analyzing subtle spectral differences between G4 conformers.

Conclusions:

  • Eps2Fold offers a rapid, inexpensive, and precise method for characterizing G-quadruplex structures from a single UV absorbance spectrum.
  • The technique facilitates G4 structural analysis, complementing existing methods like CD spectroscopy.
  • Automated analysis via the Eps2Fold web application makes G4 structural characterization more accessible.