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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 13, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Visualizing cellular G-quadruplexes with a bioinspired chemiluminescent probe.

Pengfei Wang1, Xiaoyu Li1, Zhichun Wang1

  • 1State Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.

Nucleic Acids Research
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel chemiluminescent probe (PCL) for visualizing G-quadruplexes (G4s) without external light. This method overcomes limitations of fluorescence imaging, offering high-contrast visualization in cells with minimal perturbation.

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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Chemical Biology

Background:

  • Fluorescence imaging of G-quadruplexes (G4s) faces challenges like autofluorescence and phototoxicity.
  • External excitation in fluorescence imaging limits signal-to-noise ratio and can harm live cells.
  • Chemiluminescence offers an alternative by generating light internally, bypassing excitation-induced issues.

Purpose of the Study:

  • To develop and validate the first chemiluminescent probe for selective G4 visualization.
  • To overcome the limitations of traditional fluorescence imaging for G4 detection.
  • To enable high-contrast, low-background imaging of G4s in vitro and in living cells.

Main Methods:

  • Conjugation of a G4-binding ligand (PhenDC3) with a reactive oxygen species (ROS)-activatable chemiluminescent core.
  • Design of a probe (PCL) that generates light upon ROS activation, specific to G4 environments.
  • Testing the probe's performance for G4 visualization in vitro and in live cellular imaging.

Main Results:

  • The developed probe, PCL, demonstrated selective G4 visualization.
  • PCL utilizes an excitation-free chemiluminescent mechanism for G4 imaging.
  • High-contrast and low-background imaging of G4s in living cells was achieved using PCL.
  • The chemiluminescent approach minimized cellular perturbation compared to fluorescence methods.

Conclusions:

  • PCL represents a novel chemiluminescent probe for G4 visualization.
  • This study establishes a new paradigm for G4 imaging using chemiluminescence.
  • The excitation-free mechanism provides a powerful tool for studying G4s in biological systems with reduced artifacts.