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Related Concept Videos

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Jan 13, 2026

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A Chimeric Photo-Controllable CRISPR/Cas12a System for Universal and Fast Diagnostics.

Xinrong Yan1, Bin Liu1, Shuguang Zhou2

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China.

Analytical Chemistry
|October 30, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a controllable CRISPR/Cas12a system using photo-responsive RNA. This innovation enables precise DNA detection, offering a faster, simpler, and cost-effective diagnostic tool for applications like HPV testing.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • CRISPR/Cas systems offer significant potential for biomedical applications.
  • Precise control over CRISPR/Cas activity is crucial for diagnostics and therapeutics.
  • Existing methods for CRISPR/Cas control can be complex or lack fine-tuning capabilities.

Purpose of the Study:

  • To develop a photo-controllable CRISPR/Cas12a system for enhanced diagnostic applications.
  • To demonstrate the precise regulation of CRISPR/Cas12a activity using a light-responsive crRNA.
  • To evaluate the system's efficiency and potential for clinical diagnostics.

Main Methods:

  • Conjugation of a G-quadruplex (G4) block to the 3' end of CRISPR RNA (crRNA) via a photocleavable linker.
  • Photo-irradiation to remove the G4 block, enabling crRNA-target DNA hybridization and Cas12a processing.
  • Detection of human papillomavirus 16 DNA in clinical samples using the developed one-pot strategy.

Main Results:

  • The photo-controllable CRISPR/Cas12a system demonstrated efficient detection of HPV16 DNA.
  • The system achieved high sensitivity (95.7%) and specificity (100%) compared to qPCR.
  • The method was faster (25 min vs. 60 min), simpler, and cost-effective, suitable for lateral flow tests.

Conclusions:

  • A novel, photo-controllable CRISPR/Cas12a system was successfully developed.
  • This system offers precise control over CRISPR/Cas activity for diagnostic purposes.
  • The technology holds significant promise for next-generation clinical diagnostics, particularly for infectious diseases.