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Updated: Jul 1, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Studies of sequence-specific recognition and interaction of bishairpin polyamide minor groove binders with target DNA
A S Boutorine1, W Buchmann, L Halby
1Muséum National d'Histoire Naturelle, RDDM, USM 0503, B.P. 26, 57 rue Cuvier, F-75231 Paris Cedex 05, France. alexandre.boutorine@mnhn.fr
Abstract:
The binding of bis-MGB to target DNA was studied by DNase footprint, native gel shift, circular dichroism, thermal dissociation, electrospray mass-spectrometry, and molecular modelling methods. A new method for the determination of the relative affinity of ligands against various dsDNA sequences was elaborated by using ESI-QTOF mass spectrometry. Information about affinity, sequence preferences, conformation and mode of interaction between bis-MGB and target DNA was obtained. Our experiments demonstrated that MGB have different affinity for similar cognate target sequences depending on the sequence context of the target region and other structural factors.
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