Iso-thioacetamido nucleic acids (isoTANA): synthesis and DNA binding studies of lyxo/ribo oligonucleotides
Sachin Gokhale1, Vaijayanti A Kumar
1Division of Organic Chemistry, National Chemical Laboratory, Pune 411008, India.
Abstract:
In the previous study, we developed a method to synthesize 5-atom thioacetamido linked TANA dimer blocks. Incorporation of the dimers in chimeric TANA-phosphodiester linked oligomers and the binding studies towards DNA/RNA preferences were reported. The selectivity towards RNA was found to be significantly high compared to DNA. The lyxo isomer of 3'-amino substitution in thymine was found to have higher 3'-endo conformation in monomer units. In this paper we present studies on synthesis of 3'-lyxo-3'-amino isomer of TANA, its tst and cst dimer units, and incorporation in oligomers. Preliminary results on DNA/RNA binding of these new backbone modified oligomers will be presented.
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