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Synthesis of novel fluorophores for labelling of oligonucleotides

Ajay Singh1, Ramendra K Singh

  • 1Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211002, India.

Nucleic Acids Symposium Series (2004)
|September 9, 2008
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...