Use of DNA and click chemistries to synthesize combinatorial libraries of galactosyl-phosphodiester clusters
François Morvan1, Gwladys Pourceau, Albert Meyer
1IBMM, UMR5247 CNRS Université Montpellier 1, Université Montpellier 2, Place E. Bataillon, CC1704, 34095 Montpellier Cedex 5, France. morvan@univ-montp2.fr
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
Phosphotriester polyalkyne scaffolds bearing two to four alkynes were synthesized by DNA phosphoramidite chemistry. Then two galactosyl azide derivatives exhibiting different linkers were conjugated thanks to a Copper catalyzed Alkyne Azide 1,3-dipolar cycloaddition (CuAAC) "click reaction". This reaction was performed on solid support assisted by microwaves. Thus, small libraries of 4, 8 and 16 galactosyl-clusters were obtained.


