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Updated: Jul 1, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Published on: August 12, 2019
A versatile synthetic approach for the development of libraries of 5', 3'-bis-conjugated oligonucleotides
Giovanni Di Fabio1, Cinzia Coppola, Jennifer D'Onofrio
1Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Complesso Universitario di Monte S. Angelo, via Cinthia, 4, I-80126 Napoli, Italy. giovanni.difabio@unina.it
Abstract:
A versatile approach to develop libraries of diverse 5',3'-bis-conjugated oligonucleotides (ODNs) is here described. The usage of ad hoc derivatized solid supports, to which the first nucleoside unit is attached through a phosphate linkage, opens easy synthetic access to a large variety of hybrid bis-conjugated oligomers. The G-quadruplex forming d((5')TGGGAG(3')) sequence, as a potential anti-HIV agent, has been here used as a model system.

