Synthesis and incorporation of fluorescent C-nucleoside analogue into oligodeoxyribonucleotides
Hiroaki Ozaki1, Takeshi Kawai, Masayasu Kuwahara
1Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan. h-ozaki@chem-bio.gunma-u.ac.jp
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
Fluorescent nucleoside analogues were synthesized and derived to protected nucleoside phosphoramidite. The nucleoside analogue bearing 3-aminobenzonitrile was sensitive to the surrounding environment. 3-Aminobenzonitrile is a similar size with pyrimidine base. Therefore, it is expected that the nucleoside little perturbs the formation of duplex. This nucleoside could be incorporated into DNA.
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