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Updated: Jul 1, 2026
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Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168
Naoto Ohtani1, Mitsuru Sato, Masaru Tomita
1Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
Bioscience, Biotechnology, and Biochemistry
|September 9, 2008
Abstract:
Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.
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