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Updated: Aug 11, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
Published on: April 6, 2016
Sample gating in open tubular and packed capillaries for high-speed liquid chromatography
C A Monnig1, D M Dohmeier, J W Jorgenson
1Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
Abstract:
A new liquid chromatography instrument is described that employs small-diameter capillary columns and an optically controlled sample gating procedure to separate a mixture of fluorescein isothiocyanate (FITC) labeled amines in as few as 6 s. Efficiency of the separation, expressed as the number of theoretical plates, is observed to increase linearly with column length and thus analysis time. The combination of short analysis time and automated sample introduction allows signal averaging to be used to enhance the precision of the measurement.
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