Related Experiment Video
Updated: May 5, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Continuous flow fast atom bombardment with packed microcolumns: A comparison of precolumn versus coaxial matrix
S Pleasance1, P Thibault, M A Moseley
1Atlantic Research Laboratory, National Research Council of Canada, B3H 3Z1, Halifax, Nova Scotia, Canada.
Abstract:
The effect of adding glycerol to the mobile phase on the chromatographic separation of peptides has been investigated using a continuous flow fast atom bombardment (CFFAB) interface coupled with commercial packed microcolumns (25 cm × 320 μm.i.d.). In a comparative study using a UV detector, it was found that chromatographic peak broadening progressively increased with increasing percentage of glycerol in the mobile phase. In the liquid chromatographic FAB mass spectrometric analysis, this effect is compounded by the dynamic mixing of the column effluent on the probe. Improvements of 25-155% in the overall separation efficiencies were obtained by introducing the matrix independently to the probe tip via a coaxial arrangement. Application of this coaxial CFFAB is demonstrated by the analysis of peptide mixtures and tryptic digests.
Related Concept Videos
Mass Analyzers: Common Types
Tandem Mass Spectrometry
Atomic Absorption Spectroscopy: Atomization Methods
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Principles Of Column Chromatography

