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Updated: Jul 1, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development
Quan-Sheng Huang1, Hai-Yun Wang, Peng Gao
1State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Abstract:
The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.
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