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Analysis of proteins expressed at the time of murine organogenesis
M L Van Keuren1, R A Iacob, D M Kurnit
1Department of Pediatrics, University of Michigan School of Medicine, Ann Arbor 48109-0650.
Abstract:
Two-dimensional electrophoretograms were prepared from wild-type C57BL/6J embryos from day 7.5 through day 9.0 of development. This time period encompasses a critical window of development as the embryo traverses from an egg cylinder through major organogenesis. Consequently, we term this resource MOPED (for mouse organogenesis protein electrophoresis database). By resolving and analyzing the behavior of approximately 1,000 polypeptides per time point, we were able to track many of these polypeptides through this time period in development. Of special note was a burst of induced protein synthesis that was observed in mouse embryos development. Polypeptides observed in mouse embryos that match those identified previously in mouse fibroblasts were noted. Two of them (the intermediate filament-associated protein and tropomyosin-4) were significantly altered in 8.5 day embryos. As more polypeptides are designated, it will be possible to expand the known proteins in the database. MOPED establishes the patterns of synthesis of a large number of polypeptides during a crucial period of development. Thus MOPED is designed to analyze proteins relevant to mouse embryogenesis in the future.
Insights
This study created the Mouse Organogenesis Protein Electrophoresis Database (MOPED) to track protein synthesis during critical mouse embryonic development. MOPED reveals significant protein changes during organogenesis, aiding future embryogenesis research.
Area of Science:
- Developmental Biology
- Proteomics
- Genomics
Background:
- Mouse embryonic development involves complex protein synthesis.
- Understanding protein dynamics during organogenesis is crucial.
Purpose of the Study:
- To establish a comprehensive database of proteins during mouse embryogenesis.
- To track polypeptide synthesis during a critical developmental window.
Main Methods:
- Two-dimensional electrophoresis of wild-type C57BL/6J embryos (days 7.5-9.0).
- Analysis of approximately 1,000 polypeptides per time point.
- Creation of the Mouse Organogenesis Protein Electrophoresis Database (MOPED).
Main Results:
- Identified and tracked numerous polypeptides during early mouse development.
- Observed a significant burst of induced protein synthesis.
- Noted alterations in specific proteins (intermediate filament-associated protein, tropomyosin-4) at day 8.5.
Conclusions:
- MOPED provides a valuable resource for studying protein synthesis patterns in mouse embryogenesis.
- The database facilitates future analysis of proteins critical for embryonic development.
- Identified key proteins with altered expression during organogenesis.