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Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Published on: April 27, 2011
Validation of an automated detection platform for use with the roche linear array human papillomavirus genotyping
Matthew P Stevens1, Suzanne M Garland, Sepehr N Tabrizi
1Department of Microbiology, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia. matthew.stevens@mcri.edu.au
Abstract:
An automated platform (BeeBlot) was evaluated in parallel with the recommended protocol for the hybridization and detection steps of the Roche Linear Array human papillomavirus (HPV) genotyping test using DNA from 143 cervical specimens. Genotyping profiles showed 100% concordance between the methods, suggesting that automation could complement the Roche Linear Array for enhanced speed and reproducibility.
