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Updated: Jul 1, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
Exploration of single molecule events in a haloperoxidase and its biomimic: localization of halogenation activity
Virginia Martínez Martínez1, Gert De Cremer, Maarten B J Roeffaers
1Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B-3001 Leuven, Belgium.
Abstract:
In situ observation of single oxidation/halogenation events by catalytically generated hypobromite species using single molecule fluorescence microscopy allows monitoring of the diffusion behavior of these halonium species from the catalyst into the bulk solution. The fluororgenic probe specifically reacts with hypohalites, yielding fluorescein that can be detected with single molecule sensitivity. It was found for two investigated catalysts (Curvularia verruculosa enzymes and tungstate-exchanged LDH crystals) that in steady-state conditions hypobromite is able to diffuse over 800 nm in the bulk solution before it oxidizes organic substrates.
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