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Updated: Jul 1, 2026
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Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
2,4-diacetylphloroglucinol alters plant root development
Jessica N Brazelton1, Emily E Pfeufer, Teresa A Sweat
1Biology Department, Allegheny College, Meadville, PA 16335, USA.
Molecular Plant-Microbe Interactions : MPMI
|September 13, 2008
Summary
The antibiotic 2,4-diacetylphloroglucinol (DAPG), produced by Pseudomonas fluorescens, alters crop root growth. DAPG interacts with auxin pathways, inhibiting primary root elongation and promoting lateral root formation.
Area of Science:
- Plant Pathology
- Microbial Ecology
- Biochemistry
Background:
- Pseudomonas fluorescens strains with the phlD gene produce 2,4-diacetylphloroglucinol (DAPG), an antibiotic effective against root pathogens.
- The precise mechanisms of DAPG action and its effects on plant root systems remain incompletely understood.
Purpose of the Study:
- To investigate the effects of DAPG on plant root architecture.
- To elucidate the role of auxin signaling in DAPG's impact on root development.
Main Methods:
- Assessing DAPG's impact on primary and lateral root growth in tomato seedlings.
- Examining DAPG sensitivity in auxin-resistant tomato mutants (diageotropica).
- Utilizing transgenic tobacco with a GH3 promoter::luciferase reporter to study auxin pathway responses to DAPG and P. fluorescens isolates.
Main Results:
- DAPG inhibited primary root growth and stimulated lateral root production in tomato.
- Auxin-resistant tomato mutants showed reduced sensitivity to DAPG's effects on root growth and branching.
- Exogenous DAPG and P. fluorescens supernatants suppressed auxin-inducible reporter gene activity in tobacco, with a phlD mutant showing delayed effects.
Conclusions:
- DAPG influences crop root architecture by modulating auxin-dependent signaling pathways.
- This interaction suggests a novel mechanism by which beneficial microbes can shape plant root systems.
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