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The aim of the study is to assess the dimensions of the masticatory muscles from CT scans using C2000 software and to analyse their variations depending on sagittal and vertical skeletal pattern. The maximal length, the area and the volume of the right and left masticatory muscles were measured from CT scans of 34 subjects selected upon their skeletal pattern. The analysis of the results shows that masticatory muscles dimensions vary depending on sagittal and vertical skeletal pattern. They are more developed in brachyfacial subjects and to a lesser degree in subjects without antero-posterior skeletal discrepancy. This method is time-consuming but allows a good evaluation of masticatory muscles dimensions.