Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

ALBAN (GETUG-AFU 37): a phase III, randomized, open-label international trial of intravenous atezolizumab and intravesical Bacillus Calmette-Guérin (BCG) versus BCG alone in BCG-naive high-risk, non-muscle-invasive bladder cancer (NMIBC).

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Same author

Passive Defect Driven Morphogenesis in Nematic Membranes.

Physical review letters·2025

Same author

Fluorescence angiography with indocyanine green for low anterior resection in patients with rectal cancer: a prospective before and after study.

Techniques in coloproctology·2025

Same author

Botulinum toxin injection for retrograde cricopharyngeal muscle dysfunction syndrome.

European annals of otorhinolaryngology, head and neck diseases·2024

Same author

Effect of prolactin concentration during the dry period on the subsequent milk production of dairy cows.

Journal of dairy science·2024

Same author

Trend of soil salinization in Africa and implications for agro-chemical use in semi-arid croplands.

The Science of the total environment·2024

Search research articles

Related Experiment Video

Updated: Jul 1, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Transcriptome analysis identifies genes with enriched expression in the mouse central extended amygdala.

J A J Becker¹, K Befort, C Blad

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, Département Neurobiologie et Génétique, Illkirch, France. jbecker@igbmc.u-strasbg.fr

|September 13, 2008

Summary

Researchers identified 121 genes enriched in the mouse central extended amygdala (EAc), a key brain region for reward and stress. Eight genes showed unique expression patterns, offering new molecular insights into EAc function and potential targets for manipulation.

More Related Videos

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Published on: October 10, 2012

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Related Experiment Videos

Last Updated: Jul 1, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Published on: October 10, 2012

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

The central extended amygdala (EAc) is a crucial neural network involved in reward and stress pathways, implicated in drug abuse.
Understanding EAc function at a molecular level is essential for advancing research in addiction and related neurological disorders.

Purpose of the Study:

To identify genes with enriched expression in the mouse EAc, particularly in the bed nucleus of the stria terminalis (BNST) and central nucleus of the amygdala (CeA).
To characterize the expression patterns and potential functions of these newly identified EAc-enriched genes.

Main Methods:

Genome-wide gene expression screening using Affymetrix arrays.
Non-radioactive in situ hybridization for mapping gene expression in the brain.
Quantitative Polymerase Chain Reaction (Q-PCR) for tissue-specific expression analysis.

Main Results:

Identified 121 genes with >2-fold higher expression in the EAc compared to whole brain.
Discovered 43 novel EAc-expressed genes.
Mapped eight genes with distinct rostro-caudal expression patterns across AcbSh, BNST, and CeA.
Confirmed predominant brain expression for most identified genes, including signaling proteins, a transcription factor, and proteins of unknown function.

Conclusions:

This study provides a molecular catalog of genes enriched in the EAc, expanding knowledge of this critical brain region.
The identified genes offer potential targets for future genetic manipulations and a deeper understanding of EAc's role in reward and stress.
The unique expression patterns suggest specific roles for these genes within the EAc circuitry.